rs186928850

Variant names:

• chr19-38412976-T-A
• rs186928850
• NM_170604.3:c.1490A>T

Your query was ambiguous. Multiple possible variants found:

• chr19-38412976-T-A
• chr19-38412976-T-C

Variant summary

Our verdict is Uncertain significance. Variant got 2 ACMG points: 2P and 0B. PM2

The NM_170604.3(RASGRP4):​c.1490A>T​(p.Asn497Ile) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant was absent in control chromosomes in GnomAD project. 12/21 in silico tools predict a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: not found (cov: 32)
• Consequence: RASGRP4 NM_170604.3 missense
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: 1.52

Genes affected

RASGRP4 (HGNC:18958): (RAS guanyl releasing protein 4) The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]

ACMG classification

Verdict is Uncertain_significance. Variant got 2 ACMG points.

• PM2: Very rare variant in population databases, with high coverage

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	MANE	Protein	UniProt
RASGRP4	NM_170604.3	c.1490A>T	p.Asn497Ile	missense_variant	Exon 12 of 17	ENST00000615439.5	NP_733749.1

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	TSL	MANE	Protein	Appris	UniProt
RASGRP4	ENST00000615439.5	c.1490A>T	p.Asn497Ile	missense_variant	Exon 12 of 17	1	NM_170604.3	ENSP00000479844.1
RASGRP4	ENST00000587738.2	c.1490A>T	p.Asn497Ile	missense_variant	Exon 12 of 17	5		ENSP00000465772.1
RASGRP4	ENST00000586305.5	c.1448A>T	p.Asn483Ile	missense_variant	Exon 12 of 17	1		ENSP00000467604.1
RASGRP4	ENST00000454404.6	c.1388A>T	p.Asn463Ile	missense_variant	Exon 12 of 17	1		ENSP00000416463.2
RASGRP4	ENST00000587753.5	c.1283A>T	p.Asn428Ile	missense_variant	Exon 12 of 17	1		ENSP00000468483.1
RASGRP4	ENST00000614135.4	c.1214A>T	p.Asn405Ile	missense_variant	Exon 11 of 16	5		ENSP00000479078.1
RASGRP4	ENST00000617966.4	c.1199A>T	p.Asn400Ile	missense_variant	Exon 10 of 15	5		ENSP00000479888.1
RASGRP4	ENST00000622174.4	c.923A>T	p.Asn308Ile	missense_variant	Exon 9 of 14	5		ENSP00000484345.1
RASGRP4	ENST00000589358.5	n.1490A>T		non_coding_transcript_exon_variant	Exon 12 of 18	5		ENSP00000465742.1
RASGRP4	ENST00000589474.5	n.1448A>T		non_coding_transcript_exon_variant	Exon 12 of 18	5		ENSP00000466928.1

Frequencies

GnomAD3 genomes Cov.: 32

GnomAD4 exome Cov.: 32

GnomAD4 genome Cov.: 32

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.3

Name	Calibrated prediction	Score	Prediction
AlphaMissense	Benign	0.22
BayesDel_addAF	Benign	-0.0031	T
BayesDel_noAF	Benign	-0.24
CADD	Benign	23
DANN	Uncertain	0.99
DEOGEN2	Benign	0.23	.;.;.;.;.;.;T;.;.;T;.;T
Eigen	Benign	0.16
Eigen_PC	Benign	0.13
FATHMM_MKL	Uncertain	0.91	D
LIST_S2	Uncertain	0.88	.;.;.;D;D;.;D;D;D;D;D;.
M_CAP	Benign	0.080	D
MetaRNN	Uncertain	0.74	D;D;D;D;D;D;D;D;D;D;D;D
MetaSVM	Benign	-0.64	T
MutationAssessor	Benign	1.8	.;.;.;.;.;.;.;.;.;L;.;L
PrimateAI	Benign	0.45	T
PROVEAN	Pathogenic	-6.9	.;.;.;.;.;.;.;D;D;.;D;.
REVEL	Benign	0.19
Sift	Benign	0.036	.;.;.;.;.;.;.;D;D;.;D;.
Sift4G	Uncertain	0.0020	D;D;D;D;D;D;D;D;D;D;D;D
Polyphen		0.97	D;.;.;.;.;.;.;.;.;D;.;D
Vest4		0.70
MutPred		0.53		.;.;.;.;.;.;Loss of catalytic residue at N497 (P = 0.002);.;.;Loss of catalytic residue at N497 (P = 0.002);.;Loss of catalytic residue at N497 (P = 0.002);
MVP		0.59
MPC		0.94
ClinPred		0.97	D
GERP RS		1.7
Varity_R		0.36
gMVP		0.65

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.010		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Other links and lift over

dbSNP: rs186928850; hg19: chr19-38903616;