rs201808265
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Variant summary
Our verdict is Uncertain significance. Variant got 2 ACMG points: 2P and 0B. PM2
The NM_016239.4(MYO15A):āc.4424T>Cā(p.Ile1475Thr) variant causes a missense change. The variant allele was found at a frequency of 0.000188 in 1,614,194 control chromosomes in the GnomAD database, with no homozygous occurrence. Variant has been reported in ClinVar as Conflicting classifications of pathogenicity (no stars). Synonymous variant affecting the same amino acid position (i.e. I1475I) has been classified as Likely benign.
Frequency
Genomes: š 0.00014 ( 0 hom., cov: 33)
Exomes š: 0.00019 ( 0 hom. )
Consequence
MYO15A
NM_016239.4 missense
NM_016239.4 missense
Scores
10
7
2
Clinical Significance
Conservation
PhyloP100: 6.22
Genes affected
MYO15A (HGNC:7594): (myosin XVA) This gene encodes an unconventional myosin. This protein differs from other myosins in that it has a long N-terminal extension preceding the conserved motor domain. Studies in mice suggest that this protein is necessary for actin organization in the hair cells of the cochlea. Mutations in this gene have been associated with profound, congenital, neurosensory, nonsyndromal deafness. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Read-through transcripts containing an upstream gene and this gene have been identified, but they are not thought to encode a fusion protein. Several alternatively spliced transcript variants have been described, but their full length sequences have not been determined. [provided by RefSeq, Jul 2008]
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ACMG classification
Classification made for transcript
Verdict is Uncertain_significance. Variant got 2 ACMG points.
PM2
Very rare variant in population databases, with high coverage;
Transcripts
RefSeq
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | MANE | UniProt |
---|---|---|---|---|---|---|---|
MYO15A | NM_016239.4 | c.4424T>C | p.Ile1475Thr | missense_variant | 12/66 | ENST00000647165.2 |
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | TSL | MANE | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|
MYO15A | ENST00000647165.2 | c.4424T>C | p.Ile1475Thr | missense_variant | 12/66 | NM_016239.4 | P1 |
Frequencies
GnomAD3 genomes AF: 0.000145 AC: 22AN: 152202Hom.: 0 Cov.: 33
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GnomAD3 exomes AF: 0.000301 AC: 75AN: 249420Hom.: 0 AF XY: 0.000273 AC XY: 37AN XY: 135356
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GnomAD4 exome AF: 0.000192 AC: 281AN: 1461874Hom.: 0 Cov.: 32 AF XY: 0.000191 AC XY: 139AN XY: 727234
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GnomAD4 genome AF: 0.000144 AC: 22AN: 152320Hom.: 0 Cov.: 33 AF XY: 0.000134 AC XY: 10AN XY: 74490
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ClinVar
Significance: Conflicting classifications of pathogenicity
Submissions summary: Uncertain:3Benign:1
Revision: criteria provided, conflicting classifications
LINK: link
Submissions by phenotype
not provided Uncertain:1Benign:1
Uncertain significance, criteria provided, single submitter | clinical testing | GeneDx | Aug 31, 2022 | In silico analysis supports that this missense variant has a deleterious effect on protein structure/function; Has not been previously published as pathogenic or benign to our knowledge - |
Likely benign, criteria provided, single submitter | clinical testing | Invitae | Jan 31, 2024 | - - |
not specified Uncertain:1
Uncertain significance, criteria provided, single submitter | clinical testing | Laboratory for Molecular Medicine, Mass General Brigham Personalized Medicine | May 18, 2018 | Variant classified as Uncertain Significance - Favor Benign. The p.Ile1475Thr va riant in MYO15A has been reported by our laboratory in 1 individual with unilate ral auditory neuropathy and a family history of hearing loss. This variant has a lso been reported in ClinVar (Variation ID 228953) and has been identified in 0. 12% (10/10150) of Ashkenazi Jewish chromosomes by the Genome Aggregation Databas e (gnomAD, http://gnomad.broadinstitute.org; dbSNP rs201808265). Although this v ariant has been seen in the general population, its frequency is not high enough to rule out a pathogenic role. Computational prediction tools and conservation analysis suggest that the p.Ile1475Thr variant may impact the protein, though th is information is not predictive enough to determine pathogenicity. In summary, while the clinical significance of the p.Ile1475Thr variant is uncertain, its fr equency suggests that it is more likely to be benign. ACMG/AMP criteria applied: PP3, BS1_Supporting. - |
Inborn genetic diseases Uncertain:1
Uncertain significance, criteria provided, single submitter | clinical testing | Ambry Genetics | Jun 04, 2024 | The c.4424T>C (p.I1475T) alteration is located in exon 12 (coding exon 11) of the MYO15A gene. This alteration results from a T to C substitution at nucleotide position 4424, causing the isoleucine (I) at amino acid position 1475 to be replaced by a threonine (T). Based on insufficient or conflicting evidence, the clinical significance of this alteration remains unclear. - |
Computational scores
Source:
Name
Calibrated prediction
Score
Prediction
AlphaMissense
Pathogenic
BayesDel_addAF
Uncertain
D
BayesDel_noAF
Pathogenic
CADD
Uncertain
DANN
Uncertain
DEOGEN2
Benign
T;D;D
Eigen
Pathogenic
Eigen_PC
Pathogenic
FATHMM_MKL
Pathogenic
D
LIST_S2
Uncertain
D;.;D
M_CAP
Pathogenic
D
MetaRNN
Uncertain
D;D;D
MetaSVM
Pathogenic
D
MutationAssessor
Uncertain
.;M;M
MutationTaster
Benign
D
PrimateAI
Uncertain
T
PROVEAN
Pathogenic
.;D;.
REVEL
Pathogenic
Sift
Uncertain
.;D;.
Sift4G
Pathogenic
D;D;.
Polyphen
1.0
.;D;D
Vest4
MVP
ClinPred
T
GERP RS
Varity_R
gMVP
Splicing
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SpliceAI score (max)
Details are displayed if max score is > 0.2
Find out detailed SpliceAI scores and Pangolin per-transcript scores at