rs28903092
Variant summary
Our verdict is Likely benign. Variant got -5 ACMG points: 0P and 5B. BP4_StrongBP6
The NM_005732.4(RAD50):c.2177G>A(p.Arg726His) variant causes a missense change. The variant allele was found at a frequency of 0.000133 in 1,611,854 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Conflicting classifications of pathogenicity (no stars).
Frequency
Consequence
NM_005732.4 missense
Scores
Clinical Significance
Conservation
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ACMG classification
Verdict is Likely_benign. Variant got -5 ACMG points.
Transcripts
RefSeq
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | Exon rank | TSL | MANE | Protein | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|---|
RAD50 | ENST00000378823.8 | c.2177G>A | p.Arg726His | missense_variant | Exon 13 of 25 | 1 | NM_005732.4 | ENSP00000368100.4 | ||
ENSG00000283782 | ENST00000640655.2 | c.1880G>A | p.Arg627His | missense_variant | Exon 14 of 26 | 5 | ENSP00000491596.2 |
Frequencies
GnomAD3 genomes AF: 0.000237 AC: 36AN: 152164Hom.: 0 Cov.: 32
GnomAD3 exomes AF: 0.000145 AC: 36AN: 248808Hom.: 0 AF XY: 0.000111 AC XY: 15AN XY: 134578
GnomAD4 exome AF: 0.000122 AC: 178AN: 1459572Hom.: 0 Cov.: 30 AF XY: 0.000120 AC XY: 87AN XY: 726176
GnomAD4 genome AF: 0.000236 AC: 36AN: 152282Hom.: 0 Cov.: 32 AF XY: 0.000201 AC XY: 15AN XY: 74452
ClinVar
Submissions by phenotype
Hereditary cancer-predisposing syndrome Uncertain:2Benign:1
This alteration is classified as likely benign based on a combination of the following: seen in unaffected individuals, population frequency, intact protein function, lack of segregation with disease, co-occurrence, RNA analysis, in silico models, amino acid conservation, lack of disease association in case-control studies, and/or the mechanism of disease or impacted region is inconsistent with a known cause of pathogenicity. -
This sequence change replaces arginine, which is basic and polar, with histidine, which is basic and polar, at codon 726 of the RAD50 protein (p.Arg726His). This variant is present in population databases (rs28903092, gnomAD 0.1%). This missense change has been observed in individual(s) with breast cancer (PMID: 16385572, 25503501, 32658311). ClinVar contains an entry for this variant (Variation ID: 128001). Invitae Evidence Modeling of protein sequence and biophysical properties (such as structural, functional, and spatial information, amino acid conservation, physicochemical variation, residue mobility, and thermodynamic stability) indicates that this missense variant is not expected to disrupt RAD50 protein function with a negative predictive value of 80%. In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance. -
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not provided Uncertain:2
In silico analysis supports that this missense variant does not alter protein structure/function; Variants in candidate genes are classified as variants of uncertain significance in accordance with ACMG guidelines (Richards et al., 2015); Identified in individuals with personal or family history of breast or other cancer and in an individual with primary ovarian insufficiency (Tommiska et al., 2006; Maxwell et al., 2015; Perkins et al., 2018; Tucker et al., 2019); This variant is associated with the following publications: (PMID: 29555771, 31159747, 30924587, 31980526, 16385572, 25503501) -
The RAD50 c.2177G>A (p.Arg726His) variant has been reported in the published literature in individuals with breast cancer (PMID: 32658311 (2021), 25503501 (2015), 16385572 (2006)) and in an individual undergoing hereditary cancer testing (PMID: 31159747 (2019)). In a large scale breast cancer association study, this variant has been observed in breast cancer cases, as well as reportedly healthy individuals (PMID: 33471991 (2021), see also LOVD (http://databases.lovd.nl/shared)). The frequency of this variant in the general population, 0.0011 (11/10322 chromosomes in Ashkenazi Jewish subpopulation (Genome Aggregation Database, http://gnomad.broadinstitute.org)), is higher than would generally be expected for pathogenic variants in this gene. Analysis of this variant using bioinformatics tools for the prediction of the effect of amino acid changes on protein structure and function yielded predictions that this variant is benign. Based on the available information, we are unable to determine the clinical significance of this variant. -
not specified Uncertain:1Benign:1
Variant summary: RAD50 c.2177G>A (p.Arg726His) results in a non-conservative amino acid change located in the RAD50, zinc hook domain (IPR013134) of the encoded protein sequence. Three of five in-silico tools predict a benign effect of the variant on protein function. The variant allele was found at a frequency of 0.00014 in 248808 control chromosomes (gnomAD). The observed variant frequency is approximately 2 fold of the estimated maximal expected allele frequency for a pathogenic variant in RAD50 causing Hereditary Breast And Ovarian Cancer Syndrome phenotype (6.3e-05), suggesting that the variant is benign. c.2177G>A has been reported in the literature in individuals affected with Breast Cancer (e.g. Dorling_2021, Tommiska_2006, Maxwell_2015, Tsaousis_2019, Akcay_2021) and Premature Ovarian Insufficiency (Tucker_2019). In this latter case, the patient also carried a second RAD50 variant without signs of Nijmegen Breakage Syndrome and also had a GREM1 variant (VUS) that may contribute to the Premature Ovarian Insufficiency phenotype. These reports do not provide unequivocal conclusions about association of the variant with Hereditary Breast And Ovarian Cancer Syndrome. To our knowledge, no experimental evidence demonstrating an impact on protein function has been reported. Six ClinVar submitters have assessed the variant since 2014: five classified the variant as of uncertain significance and one as likely benign. Based on the evidence outlined above, the variant was classified as likely benign. -
DNA sequence analysis of the RAD50 gene demonstrated a sequence change, c.2177G>A, in exon 13 that results in an amino acid change, p.Arg726His. This sequence change has been described in the gnomAD database with frequency of 0.11% in the Ashkenazi Jewish subpopulation (dbSNP rs28903092). The p.Arg726His change affects a moderately conserved amino acid residue located in a domain of the RAD50 protein that is known to be functional. In-silico pathogenicity prediction tools (SIFT, PolyPhen2, Align GVGD, REVEL) provide contradictory results for the p.Arg726His substitution. This sequence change has been reported in individuals who had germline genetic testing based on a personal or family history of breast cancer (PMID: 31159747, 16385572, 25503501). Due to insufficient evidences and the lack of functional studies, the clinical significance of the p.Arg726His change remains unknown at this time. -
Nijmegen breakage syndrome-like disorder Uncertain:1Other:1
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Variant classified as Uncertain significance and reported on 08-31-2017 by Invitae. GenomeConnect-Invitae Patient Insights Network assertions are reported exactly as they appear on the patient-provided report from the testing laboratory. Registry team members make no attempt to reinterpret the clinical significance of the variant. Phenotypic details are available under supporting information. -
RAD50-related disorder Uncertain:1
The RAD50 c.2177G>A variant is predicted to result in the amino acid substitution p.Arg726His. This variant has been reported in individuals with breast cancer, although its pathogenicity was not established (Tommiska et al. 2006. PubMed ID: 16385572; Maxwell et al. 2015. PubMed ID: 25503501, supplementary table 1). It was also reported as a variant of uncertain significance in individuals with a possible hereditary cancer predisposition syndrome (Perkins et al. 2018. PubMed ID: 29555771, Table S2; Tsaousis et al. 2019. PubMed ID: 31159747, Table S5) and in one individual with premature ovarian insufficiency (Tucker et al. 2019. PubMed ID: 30924587). This variant is reported in 0.11% of alleles in individuals of Ashkenazi Jewish descent in gnomAD (http://gnomad.broadinstitute.org/variant/5-131931472-G-A). In ClinVar, it has conflicting interpretations, including uncertain significance and likely benign (https://www.ncbi.nlm.nih.gov/clinvar/variation/128001/). At this time, the clinical significance of this variant is uncertain due to the absence of conclusive functional and genetic evidence. -
Premature ovarian insufficiency Uncertain:1
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Computational scores
Source:
Splicing
Find out detailed SpliceAI scores and Pangolin per-transcript scores at