rs3354

Variant names:

• chr1-94530263-C-A
• rs3354
• NM_001993.5:c.*197G>T

Your query was ambiguous. Multiple possible variants found:

• chr1-94530263-C-A
• chr1-94530263-C-G
• chr1-94530263-C-T

Variant summary

Our verdict is Likely benign. The variant received -2 ACMG points: 2P and 4B. PM2 BP4_Strong

The NM_001993.5(F3):​c.*197G>T variant causes a 3 prime UTR change involving the alteration of a non-conserved nucleotide. The variant was absent in control chromosomes in GnomAD project. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency:
  • Genomes: not found (cov: 31)
  • Exomes 𝑓: 0.0 (0 hom.)
  • Failed GnomAD Quality Control
• Consequence: F3 NM_001993.5 3_prime_UTR
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: 0.0310
• Publications: 15 publications found

Genes affected

F3 (HGNC:3541): (coagulation factor III, tissue factor) This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Aug 2020]

ACMG classification

Our verdict: Likely_benign. The variant received -2 ACMG points.

• PM2: Very rare variant in population databases, with high coverage
• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.79).

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_001993.5. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	F3	NM_001993.5	MANE Select	c.*197G>T		3_prime_UTR	Exon 6 of 6	NP_001984.1	P13726-1
	F3	NM_001178096.2		c.*208G>T		3_prime_UTR	Exon 5 of 5	NP_001171567.1	P13726-2

Ensembl Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	F3	ENST00000334047.12	TSL:1 MANE Select	c.*197G>T		3_prime_UTR	Exon 6 of 6	ENSP00000334145.7	P13726-1
	F3	ENST00000370207.4	TSL:1	c.*208G>T		3_prime_UTR	Exon 5 of 5	ENSP00000359226.4	P13726-2
	F3	ENST00000949532.1		c.*197G>T		3_prime_UTR	Exon 6 of 6	ENSP00000619591.1

Frequencies

GnomAD3 genomes Cov.: 31

GnomAD4 exome Data not reliable, filtered out with message: AC0 AF: 0.00 AC: 0 AN: 430450 Hom.: 0 Cov.: 6 AF XY: 0.00 AC XY: 0 AN XY: 222612

African (AFR) AF: 0.00 AC: 0 AN: 12502

American (AMR) AF: 0.00 AC: 0 AN: 16644

Ashkenazi Jewish (ASJ) AF: 0.00 AC: 0 AN: 12368

East Asian (EAS) AF: 0.00 AC: 0 AN: 29336

South Asian (SAS) AF: 0.00 AC: 0 AN: 31742

European-Finnish (FIN) AF: 0.00 AC: 0 AN: 26564

Middle Eastern (MID) AF: 0.00 AC: 0 AN: 2744

European-Non Finnish (NFE) AF: 0.00 AC: 0 AN: 273966

Other (OTH) AF: 0.00 AC: 0 AN: 24584

GnomAD4 genome Cov.: 31

Alfa AF: 0.00 Hom.: 2082

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.79
CADD	Benign	7.5
DANN	Benign	0.67
PhyloP100		0.031

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs3354; hg19: chr1-94995819;