rs4676410

Variant names:

• chr2-240624322-G-A
• rs4676410
• NM_001195381.3:c.89+5291G>A

Your query was ambiguous. Multiple possible variants found:

• chr2-240624322-G-A
• chr2-240624322-G-C
• chr2-240624322-G-T

Variant summary

Our verdict is Benign. The variant received -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_001195381.3(GPR35):​c.89+5291G>A variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.258 in 152,026 control chromosomes in the GnomAD database, including 5,465 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.26 (5465 hom., cov: 32)
• Consequence: GPR35 NM_001195381.3 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -3.41
• Publications: 49 publications found

Genes affected

GPR35 (HGNC:4492): (G protein-coupled receptor 35) Enables C-X-C chemokine receptor activity. Involved in several processes, including chemokine-mediated signaling pathway; negative regulation of voltage-gated calcium channel activity; and positive regulation of cytosolic calcium ion concentration. Is integral component of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

ACMG classification

Our verdict: Benign. The variant received -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.94).
• BA1: GnomAd4 highest subpopulation (AMR) allele frequency at 95% confidence interval = 0.348 is higher than 0.05.

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	MANE	Protein	UniProt
GPR35	NM_001195381.3	c.89+5291G>A		intron_variant	Intron 5 of 5		NP_001182310.1
GPR35	NM_001195382.3	c.89+5291G>A		intron_variant	Intron 5 of 5		NP_001182311.1
GPR35	NM_001394730.1	c.89+5291G>A		intron_variant	Intron 5 of 5		NP_001381659.1

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	TSL	MANE	Protein	Appris	UniProt
GPR35	ENST00000430267.2	c.89+5291G>A		intron_variant	Intron 1 of 1	5		ENSP00000411788.2
GPR35	ENST00000319838.10	c.-5+5291G>A		intron_variant	Intron 5 of 5	2		ENSP00000322731.5
GPR35	ENST00000403859.1	c.-5+5291G>A		intron_variant	Intron 5 of 5	2		ENSP00000385140.1

Frequencies

GnomAD3 genomes AF: 0.258 AC: 39210 AN: 151908 Hom.: 5449 Cov.: 32

Gnomad AFR AF: 0.329

Gnomad AMI AF: 0.270

Gnomad AMR AF: 0.355

Gnomad ASJ AF: 0.123

Gnomad EAS AF: 0.302

Gnomad SAS AF: 0.151

Gnomad FIN AF: 0.280

Gnomad MID AF: 0.146

Gnomad NFE AF: 0.202

Gnomad OTH AF: 0.230

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.258 AC: 39255 AN: 152026 Hom.: 5465 Cov.: 32 AF XY: 0.260 AC XY: 19323 AN XY: 74308

African (AFR) AF: 0.329 AC: 13660 AN: 41472

American (AMR) AF: 0.356 AC: 5433 AN: 15280

Ashkenazi Jewish (ASJ) AF: 0.123 AC: 427 AN: 3470

East Asian (EAS) AF: 0.301 AC: 1552 AN: 5150

South Asian (SAS) AF: 0.151 AC: 727 AN: 4820

European-Finnish (FIN) AF: 0.280 AC: 2956 AN: 10572

Middle Eastern (MID) AF: 0.139 AC: 41 AN: 294

European-Non Finnish (NFE) AF: 0.202 AC: 13730 AN: 67944

Other (OTH) AF: 0.228 AC: 483 AN: 2114

Alfa AF: 0.215 Hom.: 11703

Bravo AF: 0.270

Asia WGS AF: 0.224 AC: 779 AN: 3478

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.3

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.94
CADD	Benign	4.5
DANN	Benign	0.56
PhyloP100		-3.4
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs4676410; hg19: chr2-241563739;