rs7178059

Variant names:

• chr15-32729159-C-G
• rs7178059
• NM_013372.7:c.-1-1531C>G

Variant summary

Our verdict is Benign. The variant received -14 ACMG points: 0P and 14B. BP4_Strong BP6_Moderate BA1

The NM_013372.7(GREM1):​c.-1-1531C>G variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.495 in 151,356 control chromosomes in the GnomAD database, including 19,652 homozygotes. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Benign (★).

• Frequency: Genomes: 𝑓 0.50 (19652 hom., cov: 30)
• Consequence: GREM1 NM_013372.7 intron
• Clinical Significance: Benign criteria provided, single submitter B:1
• Conservation: PhyloP100: -3.19
• Publications: 2 publications found

Genes affected

GREM1 (HGNC:2001): (gremlin 1, DAN family BMP antagonist) This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

GREM1 Gene-Disease associations (from GenCC):

• hereditary mixed polyposis syndrome

  Inheritance: AD Classification: DEFINITIVE, SUPPORTIVE Submitted by: ClinGen, G2P, Orphanet
• polyposis syndrome, hereditary mixed, 1

  Inheritance: AD Classification: STRONG Submitted by: Labcorp Genetics (formerly Invitae)

ACMG classification

Our verdict: Benign. The variant received -14 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.94).
• BP6: Variant 15-32729159-C-G is Benign according to our data. Variant chr15-32729159-C-G is described in ClinVar as Benign. ClinVar VariationId is 1294095.Status of the report is criteria_provided_single_submitter, 1 stars.
• BA1: GnomAd4 highest subpopulation (NFE) allele frequency at 95% confidence interval = 0.573 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_013372.7. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	GREM1	NM_013372.7	MANE Select	c.-1-1531C>G		intron	N/A	NP_037504.1	A6XAA7
	GREM1	NM_001368719.1		c.-1-1531C>G		intron	N/A	NP_001355648.1	O60565-1
	GREM1	NM_001191323.2		c.-1-1531C>G		intron	N/A	NP_001178252.1	O60565-2

Ensembl Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	GREM1	ENST00000651154.1	MANE Select	c.-1-1531C>G		intron	N/A	ENSP00000498748.1	O60565-1
	GREM1	ENST00000560677.5	TSL:4	c.-1-1531C>G		intron	N/A	ENSP00000453387.1	H0YLY2
	GREM1	ENST00000652365.1		c.-1-1531C>G		intron	N/A	ENSP00000498763.1	O60565-1

Frequencies

GnomAD3 genomes AF: 0.496 AC: 74948 AN: 151242 Hom.: 19649 Cov.: 30

Gnomad AFR AF: 0.307

Gnomad AMI AF: 0.458

Gnomad AMR AF: 0.531

Gnomad ASJ AF: 0.584

Gnomad EAS AF: 0.553

Gnomad SAS AF: 0.540

Gnomad FIN AF: 0.571

Gnomad MID AF: 0.585

Gnomad NFE AF: 0.578

Gnomad OTH AF: 0.517

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.495 AC: 74977 AN: 151356 Hom.: 19652 Cov.: 30 AF XY: 0.498 AC XY: 36814 AN XY: 73910

African (AFR) AF: 0.306 AC: 12654 AN: 41288

American (AMR) AF: 0.531 AC: 8072 AN: 15202

Ashkenazi Jewish (ASJ) AF: 0.584 AC: 2012 AN: 3448

East Asian (EAS) AF: 0.552 AC: 2836 AN: 5140

South Asian (SAS) AF: 0.542 AC: 2599 AN: 4796

European-Finnish (FIN) AF: 0.571 AC: 5988 AN: 10478

Middle Eastern (MID) AF: 0.585 AC: 172 AN: 294

European-Non Finnish (NFE) AF: 0.578 AC: 39134 AN: 67702

Other (OTH) AF: 0.521 AC: 1093 AN: 2098

Alfa AF: 0.537 Hom.: 2773

Bravo AF: 0.483

ClinVar

ClinVar submissions

Significance: Benign

Revision: criteria provided, single submitter

Pathogenic	VUS	Benign	Condition
-	-	1	not provided (1)

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.94
CADD	Benign	0.35
DANN	Benign	0.37
PhyloP100		-3.2
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs7178059; hg19: chr15-33021360; COSMIC: COSV55714448;