rs74458693
Variant summary
Our verdict is Pathogenic. Variant got 10 ACMG points: 10P and 0B. PP3_ModeratePP5_Very_Strong
The NM_000212.3(ITGB3):c.1260G>A(p.Thr420=) variant causes a splice region, synonymous change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.00000558 in 1,613,980 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. 3/3 splice prediction tools predicting alterations to normal splicing. Variant has been reported in ClinVar as Likely pathogenic (★★★).
Frequency
Consequence
NM_000212.3 splice_region, synonymous
Scores
Clinical Significance
Conservation
Genome browser will be placed here
ACMG classification
Verdict is Pathogenic. Variant got 10 ACMG points.
Transcripts
RefSeq
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | MANE | UniProt |
---|---|---|---|---|---|---|---|
ITGB3 | NM_000212.3 | c.1260G>A | p.Thr420= | splice_region_variant, synonymous_variant | 9/15 | ENST00000559488.7 |
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | TSL | MANE | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|
ITGB3 | ENST00000559488.7 | c.1260G>A | p.Thr420= | splice_region_variant, synonymous_variant | 9/15 | 1 | NM_000212.3 | P1 | |
ITGB3 | ENST00000571680.1 | c.1260G>A | p.Thr420= | synonymous_variant | 9/9 | 1 | |||
ITGB3 | ENST00000573377.1 | c.36G>A | p.Thr12= | synonymous_variant, NMD_transcript_variant | 1/2 | 1 | |||
ITGB3 | ENST00000696963.1 | c.1260G>A | p.Thr420= | splice_region_variant, synonymous_variant | 9/14 |
Frequencies
GnomAD3 genomes ? AF: 0.00000657 AC: 1AN: 152170Hom.: 0 Cov.: 32
GnomAD3 exomes AF: 0.0000119 AC: 3AN: 251392Hom.: 0 AF XY: 0.0000221 AC XY: 3AN XY: 135860
GnomAD4 exome AF: 0.00000547 AC: 8AN: 1461810Hom.: 0 Cov.: 32 AF XY: 0.00000963 AC XY: 7AN XY: 727194
GnomAD4 genome ? AF: 0.00000657 AC: 1AN: 152170Hom.: 0 Cov.: 32 AF XY: 0.00 AC XY: 0AN XY: 74328
ClinVar
Submissions by phenotype
Glanzmann thrombasthenia Pathogenic:1
Likely pathogenic, reviewed by expert panel | curation | ClinGen Platelet Disorders Variant Curation Expert Panel, ClinGen | Nov 02, 2023 | The ITGB3 synonymous variant NM_000212.3:c.1260G>A is predicted to lead to loss of the adjacent canonical splice donor and in vitro minigene assays and sequence analysis of patient mRNA suggest the variant results in the in frame deletion of exon 9 (PMID: 8878424; PM4). This variant has been observed in homozygosity in two individuals (Patient RS in PMID: 8878424 and GT50 in PMID: 25728920; PM3), at least one of which was reported to have a phenotype specific for Glanzmann's thrombasthenia (PMID: 25728920, GT50; PP4_moderate). Furthermore, the variant is rare in control population databases (MAF<1/10,000; PM2_supporting). In summary, this variant meets criteria to be classified as likely pathogenic for GT. GT-specific criteria applied: PM3, PM4, PM2_supporting, PP3, PP4_moderate. - |
not provided Pathogenic:1
Pathogenic, criteria provided, single submitter | clinical testing | Invitae | Oct 29, 2023 | This sequence change affects codon 420 of the ITGB3 mRNA. It is a 'silent' change, meaning that it does not change the encoded amino acid sequence of the ITGB3 protein. RNA analysis indicates that this variant induces altered splicing and may result in an absent or disrupted protein product. This variant is present in population databases (rs74458693, gnomAD 0.004%). This variant has been observed in individuals with autosomal recessive Glanzmann thrombasthenia (PMID: 8878424, 25728920, 30138987). This variant is also known as position 20624G>A. ClinVar contains an entry for this variant (Variation ID: 996184). Variants that disrupt the consensus splice site are a relatively common cause of aberrant splicing (PMID: 17576681, 9536098). Studies have shown that this variant results in skipping of exon 9 and activation of a cryptic splice site and introduces a premature termination codon (PMID: 8878424). The resulting mRNA is expected to undergo nonsense-mediated decay. For these reasons, this variant has been classified as Pathogenic. - |
Computational scores
Source:
Splicing
Find out detailed SpliceAI scores and Pangolin per-transcript scores at