rs753407699

chr7-116755506-C-Achr7-116755506-C-Gchr7-116755506-C-T

Variant summary

Our verdict is Uncertain significance. Variant got 0 ACMG points: 2P and 2B. PM2 BP4_Moderate

The NM_000245.4(MET):c.1853C>A(p.Thr618Lys) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant was absent in control chromosomes in GnomAD project. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Uncertain significance (★). Another variant affecting the same amino acid position, but resulting in a different missense (i.e. T618M) has been classified as Likely benign.

• Frequency: Genomes: not found (cov: 32)
• Consequence: MET NM_000245.4 missense
• Clinical Significance: Uncertain significance criteria provided, single submitter U:1
• Conservation: PhyloP100: 3.41

Genes affected

MET (HGNC:7029): (MET proto-oncogene, receptor tyrosine kinase) This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

ACMG classification

Verdict is Uncertain_significance. Variant got 0 ACMG points.

• PM2: Very rare variant in population databases, with high coverage
• BP4: Computational evidence support a benign effect (MetaRNN=0.24099919).

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	#exon/exons	MANE	UniProt
MET	NM_000245.4	c.1853C>A	p.Thr618Lys	missense_variant	6/21	ENST00000397752.8

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	#exon/exons	TSL	MANE	Appris	UniProt
MET	ENST00000397752.8	c.1853C>A	p.Thr618Lys	missense_variant	6/21	1	NM_000245.4	P3
MET	ENST00000318493.11	c.1853C>A	p.Thr618Lys	missense_variant	6/21	1		A2
MET	ENST00000436117.3	c.1853C>A	p.Thr618Lys	missense_variant, NMD_transcript_variant	6/20	1
MET	ENST00000422097.2	c.1853C>A	p.Thr618Lys	missense_variant	6/12	3

Frequencies

GnomAD3 genomes Cov.: 32

GnomAD4 exome Cov.: 31

GnomAD4 genome Cov.: 32

ClinVar

Significance: Uncertain significance

Submissions summary: Uncertain:1

Revision: criteria provided, single submitter

Submissions by phenotype

Hereditary cancer-predisposing syndrome Uncertain:1

Uncertain significance, criteria provided, single submitter

clinical testing

Ambry Genetics

Mar 01, 2024

The p.T618K variant (also known as c.1853C>A), located in coding exon 5 of the MET gene, results from a C to A substitution at nucleotide position 1853. The threonine at codon 618 is replaced by lysine, an amino acid with similar properties. This amino acid position is well conserved in available vertebrate species. In addition, the in silico prediction for this alteration is inconclusive. Since supporting evidence is limited at this time, the clinical significance of this alteration remains unclear. -

Computational scores

Source: dbNSFP v4.3

Name	Calibrated prediction	Score	Prediction
AlphaMissense	Benign	0.12
BayesDel_addAF	Uncertain	0.12	D
BayesDel_noAF	Uncertain	-0.060
Cadd	Benign	20
Dann	Uncertain	0.98
DEOGEN2	Uncertain	0.43	T;.;.
Eigen	Uncertain	0.29
Eigen_PC	Uncertain	0.36
FATHMM_MKL	Uncertain	0.82	D
LIST_S2	Uncertain	0.87	D;D;D
M_CAP	Benign	0.046	D
MetaRNN	Benign	0.24	T;T;T
MetaSVM	Uncertain	-0.16	T
MutationAssessor	Benign	1.9	M;M;M
MutationTaster	Benign	0.84	N;N;N
PrimateAI	Benign	0.47	T
PROVEAN	Benign	-0.63	N;N;N
REVEL	Uncertain	0.38
Sift	Uncertain	0.014	D;D;T
Sift4G	Benign	0.19	T;T;T
Polyphen		0.63	P;P;.
Vest4		0.39
MutPred		0.50		Gain of ubiquitination at T618 (P = 0.0066);Gain of ubiquitination at T618 (P = 0.0066);Gain of ubiquitination at T618 (P = 0.0066);
MVP		0.80
MPC		0.71
ClinPred		0.78	D
GERP RS		5.9
Varity_R		0.22
gMVP		0.77

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

No publications associated with this variant yet.

Other links and lift over

hg19: chr7-116395560; COSMIC: COSV100577066; COSMIC: COSV100577066;