rs797044578
Variant summary
Our verdict is Pathogenic. Variant got 16 ACMG points: 16P and 0B. PM1PM2PP3_StrongPP5_Very_Strong
The NM_002055.5(GFAP):c.772C>T(p.Arg258Cys) variant causes a missense change. The variant was absent in control chromosomes in GnomAD project. In-silico tool predicts a pathogenic outcome for this variant. Variant has been reported in ClinVar as Pathogenic (★★). Another variant affecting the same amino acid position, but resulting in a different missense (i.e. R258P) has been classified as Uncertain significance.
Frequency
Genomes: not found (cov: 33)
Consequence
GFAP
NM_002055.5 missense
NM_002055.5 missense
Scores
7
4
2
Clinical Significance
Conservation
PhyloP100: 3.90
Genes affected
GFAP (HGNC:4235): (glial fibrillary acidic protein) This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
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ACMG classification
Classification made for transcript
Verdict is Pathogenic. Variant got 16 ACMG points.
PM1
?
In a hotspot region, there are 2 aminoacids with missense pathogenic changes in the window of +-8 aminoacids around while only 0 benign, 7 uncertain in NM_002055.5
PM2
?
Very rare variant in population databases, with high coverage;
PP3
?
MetaRNN computational evidence supports a deleterious effect, 0.975
PP5
?
Variant 17-44913277-G-A is Pathogenic according to our data. Variant chr17-44913277-G-A is described in ClinVar as [Pathogenic]. Clinvar id is 190345.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars.
Transcripts
RefSeq
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | MANE | UniProt |
---|---|---|---|---|---|---|---|
GFAP | NM_002055.5 | c.772C>T | p.Arg258Cys | missense_variant | 4/9 | ENST00000588735.3 | |
GFAP | NM_001363846.2 | c.772C>T | p.Arg258Cys | missense_variant | 4/10 | ||
GFAP | NM_001242376.3 | c.772C>T | p.Arg258Cys | missense_variant | 4/7 | ||
GFAP | NM_001131019.3 | c.772C>T | p.Arg258Cys | missense_variant | 4/8 |
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | TSL | MANE | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|
GFAP | ENST00000588735.3 | c.772C>T | p.Arg258Cys | missense_variant | 4/9 | 1 | NM_002055.5 | P1 |
Frequencies
GnomAD3 genomes ? Cov.: 33
GnomAD3 genomes
?
Cov.:
33
GnomAD4 exome Cov.: 32
GnomAD4 exome
Cov.:
32
GnomAD4 genome ? Cov.: 33
GnomAD4 genome
?
Cov.:
33
ClinVar
Significance: Pathogenic
Submissions summary: Pathogenic:2Other:1
Revision: criteria provided, multiple submitters, no conflicts
LINK: link
Submissions by phenotype
Alexander disease Pathogenic:1Other:1
not provided, no classification provided | literature only | GeneReviews | - | - - |
Pathogenic, criteria provided, single submitter | clinical testing | Victorian Clinical Genetics Services, Murdoch Childrens Research Institute | May 12, 2020 | A heterozygous missense variant, NM_002055.4(GFAP):c.772C>T, has been identified in exon 4 of 9 of the GFAP gene. The variant is predicted to result in a major amino acid change from arginine to cysteine at position 258 of the protein (NP_002046.1:p.Arg258Cys). The arginine residue at this position has moderate conservation (100 vertebrates, UCSC), and is located within Coil 2A of the rod functional domain. In silico predictions for this variant are consistently pathogenic (Polyphen, SIFT, CADD, Mutation Taster). The variant is absent in the gnomAD population database. This variant has been reported as pathogenic in multiple patients with Alexander disease (ClinVar, Jost, M., et al. (2017)). Additionally, transfected HeLa and US-OS cells showed abnormal protein aggregates, where significantly more cells contained these aggregates compared to controls (Tulyeu, J., et al. (2019)). A different variant in the same codon resulting in a change to proline, has also been reported in a patient with Alexander disease (ClinVar, Brenner, M., et al . (2001)). Based on the information available at the time of curation, this variant has been classified as PATHOGENIC. - |
not provided Pathogenic:1
Pathogenic, criteria provided, single submitter | clinical testing | Athena Diagnostics | Feb 01, 2022 | This variant has not been reported in large, multi-ethnic general populations (http://gnomad.broadinstitute.org). This variant has been identified in multiple unrelated individuals with clinical features associated with this gene, including at least one apparent de novo. Assessment of experimental evidence suggests this variant results in abnormal protein function. This variant showed protein aggregate formation in vitro in multiple human cell types (PMID: 30213442). - |
Computational scores
Source:
Name
Calibrated prediction
Score
Prediction
AlphaMissense
Benign
BayesDel_addAF
Pathogenic
D
BayesDel_noAF
Pathogenic
Cadd
Pathogenic
Dann
Pathogenic
Eigen
Uncertain
Eigen_PC
Uncertain
FATHMM_MKL
Uncertain
D
LIST_S2
Pathogenic
D;D;D;D;D;D;D;D;D
M_CAP
Pathogenic
D
MetaRNN
Pathogenic
D;D;D;D;D;D;D;D;D
MetaSVM
Pathogenic
D
MutationTaster
Benign
D;D;D;D
PrimateAI
Uncertain
T
Polyphen
1.0
.;D;.;.;.;.;.;.;.
Vest4
0.80, 0.81, 0.88
MutPred
Loss of disorder (P = 0.0294);Loss of disorder (P = 0.0294);Loss of disorder (P = 0.0294);.;Loss of disorder (P = 0.0294);Loss of disorder (P = 0.0294);Loss of disorder (P = 0.0294);.;.;
MVP
1.0
MPC
1.4
ClinPred
D
GERP RS
Varity_R
gMVP
Splicing
Name
Calibrated prediction
Score
Prediction
SpliceAI score (max)
Details are displayed if max score is > 0.2
Find out detailed SpliceAI scores and Pangolin per-transcript scores at