rs873456

Positions:

• chr5-35199555-A-C

Variant summary

Our verdict is Benign. Variant got -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_000949.7(PRLR):​c.-106+30713T>G variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.567 in 151,368 control chromosomes in the GnomAD database, including 25,562 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.57 (25562 hom., cov: 31)
• Consequence: PRLR NM_000949.7 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: 0.0360

Genes affected

PRLR (HGNC:9446): (prolactin receptor) This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011]

ACMG classification

Verdict is Benign. Variant got -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.84).
• BA1: GnomAd4 highest subpopulation (NFE) allele frequency at 95% confidence interval = 0.665 is higher than 0.05.

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	#exon/exons	MANE	UniProt
PRLR	NM_000949.7	c.-106+30713T>G		intron_variant		ENST00000618457.5
PRLR	XM_024446131.2	c.59+30713T>G		intron_variant

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	#exon/exons	TSL	MANE	Appris	UniProt
PRLR	ENST00000618457.5	c.-106+30713T>G		intron_variant		1	NM_000949.7	P1
PRLR	ENST00000504500.5	c.-293+30713T>G		intron_variant		3
PRLR	ENST00000515839.1	c.-268-3842T>G		intron_variant		2
PRLR	ENST00000508107.5	c.-106+30713T>G		intron_variant, NMD_transcript_variant		3

Frequencies

GnomAD3 genomes AF: 0.567 AC: 85824 AN: 151250 Hom.: 25559 Cov.: 31

Gnomad AFR AF: 0.407

Gnomad AMI AF: 0.695

Gnomad AMR AF: 0.553

Gnomad ASJ AF: 0.636

Gnomad EAS AF: 0.281

Gnomad SAS AF: 0.658

Gnomad FIN AF: 0.615

Gnomad MID AF: 0.627

Gnomad NFE AF: 0.670

Gnomad OTH AF: 0.568

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.567 AC: 85853 AN: 151368 Hom.: 25562 Cov.: 31 AF XY: 0.567 AC XY: 41898 AN XY: 73936

Gnomad4 AFR AF: 0.407

Gnomad4 AMR AF: 0.552

Gnomad4 ASJ AF: 0.636

Gnomad4 EAS AF: 0.281

Gnomad4 SAS AF: 0.657

Gnomad4 FIN AF: 0.615

Gnomad4 NFE AF: 0.670

Gnomad4 OTH AF: 0.570

Alfa AF: 0.648 Hom.: 38978

Bravo AF: 0.549

Asia WGS AF: 0.485 AC: 1682 AN: 3476

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.3

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.84
CADD	Benign	4.1
DANN	Benign	0.68

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Other links and lift over

dbSNP: rs873456; hg19: chr5-35199657;