chr19-38410968-G-C

Variant summary

Our verdict is Uncertain significance. Variant got 1 ACMG points: 2P and 1B. PM2BP4

The NM_170604.3(RASGRP4):ā€‹c.1883C>Gā€‹(p.Ser628Cys) variant causes a missense change. The variant allele was found at a frequency of 0.00000276 in 1,449,928 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. 12/21 in silico tools predict a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

Frequency

Genomes: not found (cov: 32)
Exomes š‘“: 0.0000028 ( 0 hom. )

Consequence

RASGRP4
NM_170604.3 missense

Scores

7
12

Clinical Significance

Not reported in ClinVar

Conservation

PhyloP100: 3.83
Variant links:
Genes affected
RASGRP4 (HGNC:18958): (RAS guanyl releasing protein 4) The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]

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ACMG classification

Classification made for transcript

Verdict is Uncertain_significance. Variant got 1 ACMG points.

PM2
Very rare variant in population databases, with high coverage;
BP4
Computational evidence support a benign effect (MetaRNN=0.27219382).

Transcripts

RefSeq

Gene Transcript HGVSc HGVSp Effect Exon rank MANE Protein UniProt
RASGRP4NM_170604.3 linkc.1883C>G p.Ser628Cys missense_variant Exon 16 of 17 ENST00000615439.5 NP_733749.1 Q8TDF6-1

Ensembl

Gene Transcript HGVSc HGVSp Effect Exon rank TSL MANE Protein Appris UniProt
RASGRP4ENST00000615439.5 linkc.1883C>G p.Ser628Cys missense_variant Exon 16 of 17 1 NM_170604.3 ENSP00000479844.1 Q8TDF6-1
RASGRP4ENST00000587738.2 linkc.1883C>G p.Ser628Cys missense_variant Exon 16 of 17 5 ENSP00000465772.1 Q8TDF6-1
RASGRP4ENST00000586305.5 linkc.1841C>G p.Ser614Cys missense_variant Exon 16 of 17 1 ENSP00000467604.1 Q8TDF6-2
RASGRP4ENST00000454404.6 linkc.1781C>G p.Ser594Cys missense_variant Exon 16 of 17 1 ENSP00000416463.2 Q8TDF6-8
RASGRP4ENST00000587753.5 linkc.1676C>G p.Ser559Cys missense_variant Exon 16 of 17 1 ENSP00000468483.1 Q8TDF6-9
RASGRP4ENST00000614135.4 linkc.1607C>G p.Ser536Cys missense_variant Exon 15 of 16 5 ENSP00000479078.1 Q8TDF6-5
RASGRP4ENST00000617966.4 linkc.1592C>G p.Ser531Cys missense_variant Exon 14 of 15 5 ENSP00000479888.1 Q8TDF6-7
RASGRP4ENST00000622174.4 linkc.1316C>G p.Ser439Cys missense_variant Exon 13 of 14 5 ENSP00000484345.1 Q8TDF6-6
RASGRP4ENST00000589358.5 linkn.1883C>G non_coding_transcript_exon_variant Exon 16 of 18 5 ENSP00000465742.1 Q8TDF6-1
RASGRP4ENST00000589474.5 linkn.1841C>G non_coding_transcript_exon_variant Exon 16 of 18 5 ENSP00000466928.1 Q8TDF6-2

Frequencies

GnomAD3 genomes
Cov.:
32
GnomAD3 exomes
AF:
0.00000883
AC:
2
AN:
226382
Hom.:
0
AF XY:
0.00000817
AC XY:
1
AN XY:
122448
show subpopulations
Gnomad AFR exome
AF:
0.00
Gnomad AMR exome
AF:
0.00
Gnomad ASJ exome
AF:
0.00
Gnomad EAS exome
AF:
0.00
Gnomad SAS exome
AF:
0.00
Gnomad FIN exome
AF:
0.00
Gnomad NFE exome
AF:
0.00000983
Gnomad OTH exome
AF:
0.000179
GnomAD4 exome
AF:
0.00000276
AC:
4
AN:
1449928
Hom.:
0
Cov.:
31
AF XY:
0.00000417
AC XY:
3
AN XY:
720036
show subpopulations
Gnomad4 AFR exome
AF:
0.00
Gnomad4 AMR exome
AF:
0.00
Gnomad4 ASJ exome
AF:
0.00
Gnomad4 EAS exome
AF:
0.00
Gnomad4 SAS exome
AF:
0.00
Gnomad4 FIN exome
AF:
0.00
Gnomad4 NFE exome
AF:
0.00000361
Gnomad4 OTH exome
AF:
0.00
GnomAD4 genome
Cov.:
32
ExAC
AF:
0.00000828
AC:
1

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.3

Name
Calibrated prediction
Score
Prediction
AlphaMissense
Benign
0.070
BayesDel_addAF
Benign
-0.025
T
BayesDel_noAF
Benign
-0.25
CADD
Benign
23
DANN
Uncertain
0.99
DEOGEN2
Benign
0.037
.;.;.;.;.;.;.;.;T;.;T
Eigen
Uncertain
0.36
Eigen_PC
Uncertain
0.42
FATHMM_MKL
Uncertain
0.78
D
LIST_S2
Benign
0.83
.;.;.;T;T;.;T;T;T;T;.
M_CAP
Benign
0.035
D
MetaRNN
Benign
0.27
T;T;T;T;T;T;T;T;T;T;T
MetaSVM
Benign
-0.35
T
MutationAssessor
Benign
0.90
.;.;.;.;.;.;.;.;L;.;L
PrimateAI
Benign
0.34
T
PROVEAN
Uncertain
-2.5
.;.;.;.;.;.;D;D;.;N;.
REVEL
Benign
0.28
Sift
Uncertain
0.0020
.;.;.;.;.;.;D;D;.;D;.
Sift4G
Uncertain
0.054
T;T;T;T;T;D;D;T;T;T;T
Polyphen
0.91
P;.;.;.;.;.;.;.;P;.;P
Vest4
0.19
MutPred
0.28
.;.;.;.;.;.;.;.;Gain of catalytic residue at L629 (P = 0.0235);.;Gain of catalytic residue at L629 (P = 0.0235);
MVP
0.80
MPC
0.28
ClinPred
0.68
D
GERP RS
5.4
Varity_R
0.29
gMVP
0.47

Splicing

Name
Calibrated prediction
Score
Prediction
SpliceAI score (max)
0.0
Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Below is the list of publications found by LitVar. It may be empty.

Other links and lift over

dbSNP: rs755325515; hg19: chr19-38901608; API