chr6-29672639-G-C

Variant summary

Our verdict is Benign. Variant got -13 ACMG points: 0P and 13B. BP4_StrongBP6BS1BS2

The NM_001109809.5(ZFP57):ā€‹c.1472C>Gā€‹(p.Thr491Ser) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.00135 in 1,611,308 control chromosomes in the GnomAD database, including 13 homozygotes. In-silico tool predicts a benign outcome for this variant. 14/20 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Conflicting classifications of pathogenicity (no stars).

Frequency

Genomes: š‘“ 0.0022 ( 8 hom., cov: 32)
Exomes š‘“: 0.0013 ( 5 hom. )

Consequence

ZFP57
NM_001109809.5 missense

Scores

18

Clinical Significance

Conflicting classifications of pathogenicity criteria provided, conflicting classifications U:2B:4

Conservation

PhyloP100: 0.434
Variant links:
Genes affected
ZFP57 (HGNC:18791): (ZFP57 zinc finger protein) The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).[provided by RefSeq, Sep 2009]

Genome browser will be placed here

ACMG classification

Classification made for transcript

Verdict is Benign. Variant got -13 ACMG points.

BP4
Computational evidence support a benign effect (MetaRNN=0.0045357347).
BP6
Variant 6-29672639-G-C is Benign according to our data. Variant chr6-29672639-G-C is described in ClinVar as [Conflicting_classifications_of_pathogenicity]. Clinvar id is 356211.We mark this variant Likely_benign, oryginal submissions are: {Likely_benign=3, Uncertain_significance=2}. Variant chr6-29672639-G-C is described in Lovd as [Likely_benign].
BS1
Variant frequency is greater than expected in population amr. gnomad4 allele frequency = 0.0022 (335/152254) while in subpopulation AMR AF= 0.00642 (98/15276). AF 95% confidence interval is 0.00539. There are 8 homozygotes in gnomad4. There are 180 alleles in male gnomad4 subpopulation. Median coverage is 32. This position pass quality control queck.
BS2
High AC in GnomAd4 at 335 AD gene.

Transcripts

RefSeq

Gene Transcript HGVSc HGVSp Effect #exon/exons MANE UniProt
ZFP57NM_001109809.5 linkuse as main transcriptc.1472C>G p.Thr491Ser missense_variant 5/5 ENST00000376883.2
ZFP57NM_001366333.2 linkuse as main transcriptc.1256C>G p.Thr419Ser missense_variant 4/4

Ensembl

Gene Transcript HGVSc HGVSp Effect #exon/exons TSL MANE Appris UniProt
ZFP57ENST00000376883.2 linkuse as main transcriptc.1472C>G p.Thr491Ser missense_variant 5/55 NM_001109809.5 P1Q9NU63-3
ZFP57ENST00000488757.6 linkuse as main transcriptc.1256C>G p.Thr419Ser missense_variant 4/41

Frequencies

GnomAD3 genomes
AF:
0.00216
AC:
328
AN:
152136
Hom.:
7
Cov.:
32
show subpopulations
Gnomad AFR
AF:
0.00196
Gnomad AMI
AF:
0.00
Gnomad AMR
AF:
0.00642
Gnomad ASJ
AF:
0.000288
Gnomad EAS
AF:
0.000578
Gnomad SAS
AF:
0.000207
Gnomad FIN
AF:
0.000377
Gnomad MID
AF:
0.0158
Gnomad NFE
AF:
0.00185
Gnomad OTH
AF:
0.00431
GnomAD3 exomes
AF:
0.00153
AC:
375
AN:
244546
Hom.:
2
AF XY:
0.00158
AC XY:
212
AN XY:
133844
show subpopulations
Gnomad AFR exome
AF:
0.00105
Gnomad AMR exome
AF:
0.00204
Gnomad ASJ exome
AF:
0.000101
Gnomad EAS exome
AF:
0.00258
Gnomad SAS exome
AF:
0.000329
Gnomad FIN exome
AF:
0.0000929
Gnomad NFE exome
AF:
0.00195
Gnomad OTH exome
AF:
0.00267
GnomAD4 exome
AF:
0.00126
AC:
1834
AN:
1459054
Hom.:
5
Cov.:
31
AF XY:
0.00127
AC XY:
924
AN XY:
725444
show subpopulations
Gnomad4 AFR exome
AF:
0.00203
Gnomad4 AMR exome
AF:
0.00222
Gnomad4 ASJ exome
AF:
0.000192
Gnomad4 EAS exome
AF:
0.00134
Gnomad4 SAS exome
AF:
0.000255
Gnomad4 FIN exome
AF:
0.0000956
Gnomad4 NFE exome
AF:
0.00127
Gnomad4 OTH exome
AF:
0.00171
GnomAD4 genome
AF:
0.00220
AC:
335
AN:
152254
Hom.:
8
Cov.:
32
AF XY:
0.00242
AC XY:
180
AN XY:
74424
show subpopulations
Gnomad4 AFR
AF:
0.00214
Gnomad4 AMR
AF:
0.00642
Gnomad4 ASJ
AF:
0.000288
Gnomad4 EAS
AF:
0.000579
Gnomad4 SAS
AF:
0.000207
Gnomad4 FIN
AF:
0.000377
Gnomad4 NFE
AF:
0.00185
Gnomad4 OTH
AF:
0.00426
Alfa
AF:
0.00185
Hom.:
1
Bravo
AF:
0.00230
TwinsUK
AF:
0.00108
AC:
4
ALSPAC
AF:
0.00130
AC:
5
ESP6500AA
AF:
0.00118
AC:
3
ESP6500EA
AF:
0.00195
AC:
10
ExAC
AF:
0.00171
AC:
199
Asia WGS
AF:
0.00318
AC:
11
AN:
3478
EpiCase
AF:
0.00278
EpiControl
AF:
0.00302

ClinVar

Significance: Conflicting classifications of pathogenicity
Submissions summary: Uncertain:2Benign:4
Revision: criteria provided, conflicting classifications
LINK: link

Submissions by phenotype

not provided Benign:2
Likely benign, criteria provided, single submitterclinical testingCeGaT Center for Human Genetics TuebingenSep 01, 2023ZFP57: BP4, BS2 -
Likely benign, criteria provided, single submitterclinical testingInvitaeDec 28, 2023- -
Inborn genetic diseases Uncertain:1
Uncertain significance, criteria provided, single submitterclinical testingAmbry GeneticsJul 20, 2021The c.1472C>G (p.T491S) alteration is located in exon 4 (coding exon 4) of the ZFP57 gene. This alteration results from a C to G substitution at nucleotide position 1472, causing the threonine (T) at amino acid position 491 to be replaced by a serine (S). Based on insufficient or conflicting evidence, the clinical significance of this alteration remains unclear. -
Monogenic diabetes Uncertain:1
Uncertain significance, criteria provided, single submitterresearchPersonalized Diabetes Medicine Program, University of Maryland School of MedicineFeb 01, 2019ACMG criteria: BP4 (Revel score 0.024 + 9 predictors)=VUS -
Diabetes mellitus, transient neonatal, 1 Benign:1
Likely benign, criteria provided, single submitterclinical testingIllumina Laboratory Services, IlluminaJan 12, 2018This variant was observed in the ICSL laboratory as part of a predisposition screen in an ostensibly healthy population. It had not been previously curated by ICSL or reported in the Human Gene Mutation Database (HGMD: prior to June 1st, 2018), and was therefore a candidate for classification through an automated scoring system. Utilizing variant allele frequency, disease prevalence and penetrance estimates, and inheritance mode, an automated score was calculated to assess if this variant is too frequent to cause the disease. Based on the score and internal cut-off values, a variant classified as likely benign is not then subjected to further curation. The score for this variant resulted in a classification of likely benign for this disease. -
ZFP57-related disorder Benign:1
Likely benign, criteria provided, single submitterclinical testingPreventionGenetics, part of Exact SciencesSep 19, 2019This variant is classified as likely benign based on ACMG/AMP sequence variant interpretation guidelines (Richards et al. 2015 PMID: 25741868, with internal and published modifications). -

Computational scores

Source: dbNSFP v4.3

Name
Calibrated prediction
Score
Prediction
AlphaMissense
Benign
0.10
BayesDel_addAF
Benign
-0.70
T
BayesDel_noAF
Benign
-0.78
CADD
Benign
1.2
DANN
Benign
0.46
DEOGEN2
Benign
0.030
.;.;T
Eigen
Benign
-1.0
Eigen_PC
Benign
-1.0
FATHMM_MKL
Benign
0.072
N
LIST_S2
Benign
0.43
T;T;T
M_CAP
Benign
0.0019
T
MetaRNN
Benign
0.0045
T;T;T
MetaSVM
Benign
-0.92
T
MutationTaster
Benign
1.0
N;N;N
PrimateAI
Benign
0.23
T
PROVEAN
Benign
0.0
N;N;.
REVEL
Benign
0.024
Sift
Benign
0.71
T;T;.
Sift4G
Benign
0.72
T;T;T
Polyphen
0.0010
B;B;.
Vest4
0.10
MutPred
0.40
Gain of disorder (P = 0.0423);.;.;
MVP
0.15
MPC
0.38
ClinPred
0.00054
T
GERP RS
2.1
Varity_R
0.030
gMVP
0.038

Splicing

Name
Calibrated prediction
Score
Prediction
SpliceAI score (max)
0.0
Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Below is the list of publications found by LitVar. It may be empty.

Other links and lift over

dbSNP: rs184974475; hg19: chr6-29640416; API