rs182345541
Variant summary
Our verdict is Likely benign. Variant got -5 ACMG points: 0P and 5B. BP4_StrongBS1_Supporting
The NM_178138.6(LHX3):c.920G>C(p.Arg307Pro) variant causes a missense change. The variant allele was found at a frequency of 0.00138 in 1,548,308 control chromosomes in the GnomAD database, including 2 homozygotes. In-silico tool predicts a benign outcome for this variant. 12/20 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Conflicting classifications of pathogenicity (no stars). Synonymous variant affecting the same amino acid position (i.e. R307R) has been classified as Likely benign.
Frequency
Consequence
NM_178138.6 missense
Scores
Clinical Significance
Conservation
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ACMG classification
Verdict is Likely_benign. Variant got -5 ACMG points.
Transcripts
RefSeq
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | MANE | UniProt |
---|---|---|---|---|---|---|---|
LHX3 | NM_178138.6 | c.920G>C | p.Arg307Pro | missense_variant | 6/6 | ENST00000371748.10 | |
LHX3 | NM_014564.5 | c.935G>C | p.Arg312Pro | missense_variant | 6/6 | ||
LHX3 | NM_001363746.1 | c.887G>C | p.Arg296Pro | missense_variant | 6/6 | ||
LHX3 | XM_017015168.1 | c.848G>C | p.Arg283Pro | missense_variant | 6/6 |
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | TSL | MANE | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|
LHX3 | ENST00000371748.10 | c.920G>C | p.Arg307Pro | missense_variant | 6/6 | 1 | NM_178138.6 | ||
LHX3 | ENST00000371746.9 | c.935G>C | p.Arg312Pro | missense_variant | 6/6 | 1 | P1 | ||
LHX3 | ENST00000619587.1 | c.887G>C | p.Arg296Pro | missense_variant | 6/6 | 1 | |||
LHX3 | ENST00000645419.1 | n.1745G>C | non_coding_transcript_exon_variant | 5/5 |
Frequencies
GnomAD3 genomes ? AF: 0.000743 AC: 113AN: 152104Hom.: 0 Cov.: 33
GnomAD3 exomes AF: 0.000652 AC: 99AN: 151868Hom.: 0 AF XY: 0.000557 AC XY: 46AN XY: 82636
GnomAD4 exome AF: 0.00145 AC: 2021AN: 1396086Hom.: 2 Cov.: 34 AF XY: 0.00145 AC XY: 998AN XY: 687896
GnomAD4 genome ? AF: 0.000742 AC: 113AN: 152222Hom.: 0 Cov.: 33 AF XY: 0.000645 AC XY: 48AN XY: 74420
ClinVar
Submissions by phenotype
not provided Uncertain:2
Uncertain significance, criteria provided, single submitter | clinical testing | Invitae | Mar 04, 2022 | This sequence change replaces arginine, which is basic and polar, with proline, which is neutral and non-polar, at codon 312 of the LHX3 protein (p.Arg312Pro). This variant is present in population databases (rs182345541, gnomAD 0.1%), and has an allele count higher than expected for a pathogenic variant. This variant has not been reported in the literature in individuals affected with LHX3-related conditions. ClinVar contains an entry for this variant (Variation ID: 279837). Algorithms developed to predict the effect of missense changes on protein structure and function are either unavailable or do not agree on the potential impact of this missense change (SIFT: "Not Available"; PolyPhen-2: "Benign"; Align-GVGD: "Not Available"). In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance. - |
Uncertain significance, criteria provided, single submitter | clinical testing | GeneDx | Jul 20, 2016 | The R312P variant in the LHX3 gene has been reported previously in one individual with pituitary aplasia; however, no second LHX3 variant was identified, and R312P was also present in the heterozygous state in this individual's unaffected father and sister (Sobrier et al., 2006). The R312P variant was not observed at any significant frequency in approximately 6300 individuals of European and African American ancestry in the NHLBI Exome Sequencing Project, indicating it is not a common benign variant in these populations. The R312P variant is a non-conservative amino acid substitution, which is likely to impact secondary protein structure as these residues differ in polarity, charge, size and/or other properties. However, this substitution occurs at a position that is not conserved, and in silico analysis predicts this variant likely does not alter the protein structure/function. We interpret R312P as a variant of uncertain significance. - |
Non-acquired combined pituitary hormone deficiency with spine abnormalities Uncertain:1Benign:1
Uncertain significance, criteria provided, single submitter | clinical testing | Fulgent Genetics, Fulgent Genetics | Oct 31, 2018 | - - |
Likely benign, criteria provided, single submitter | clinical testing | Illumina Laboratory Services, Illumina | Apr 27, 2017 | This variant was observed as part of a predisposition screen in an ostensibly healthy population. A literature search was performed for the gene, cDNA change, and amino acid change (where applicable). Publications were found based on this search. The evidence from the literature, in combination with allele frequency data from public databases where available, was sufficient to determine this variant is unlikely to cause disease. Therefore, this variant is classified as likely benign. - |
Combined pituitary hormone deficiencies, genetic form Uncertain:1
Uncertain significance, no assertion criteria provided | clinical testing | Natera, Inc. | Dec 24, 2019 | - - |
Computational scores
Source:
Splicing
Find out detailed SpliceAI scores and Pangolin per-transcript scores at